Which microscope inverts the image




















This inversion is a result of the path of the light rays, which ultimately transfer the image to the eye. Most compound microscopes contain an illuminator system built into the base of the microscope.

The light generated by the bulb in the illuminator system passes through a blue filter and is collected in the microscope's substage condenser, which is the flat-bottomed panel at the bottom of a microscope. It forms an upside-down and magnified image called a real image because the light rays actually pass through the place where the image lies. The ocular lens , or eyepiece lens, acts as a magnifying glass for this real image.

The ocular lens makes the light rays spread more, so that they appear to come from a large inverted image beyond the objective lens. Because light rays do not actually pass through this location, the image is called a virtual image. The most important consideration for image formation with the objective lens other than its magnification or power is its numerical aperture.

This is a number that is directly related to the resolving power of the objective. Digital microscopes that project images onto a screen correct for this problem, but laboratory-grade compound microscopes invert images, meaning they are upside down to the observer. The inverted image is made from a positive lens, which means the image formed after light passes through the lens is a real image.

This real image is inverted at the focal length. Figure 3. These uterine cervix cells, viewed through a light microscope, were obtained from a Pap smear. Normal cells are on the left. The cells on the right are infected with human papillomavirus HPV. Notice that the infected cells are larger; also, two of these cells each have two nuclei instead of one, the normal number. Have you ever heard of a medical test called a Pap smear shown in Figure 3?

In this test, a doctor takes a small sample of cells from the uterine cervix of a patient and sends it to a medical lab where a cytotechnologist stains the cells and examines them for any changes that could indicate cervical cancer or a microbial infection. They are trained to determine which cellular changes are within normal limits and which are abnormal.

Their focus is not limited to cervical cells; they study cellular specimens that come from all organs. When they notice abnormalities, they consult a pathologist, who is a medical doctor who can make a clinical diagnosis.

A cell is the smallest unit of life. Most cells are so tiny that they cannot be seen with the naked eye. Therefore, scientists use microscopes to study cells.



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